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Phosphorylation of JAK/STAT and MAPKs by SeviL in RAW264.7 cells. Cells (5 × 10 5 ) were treated with SeviL (10 μg/mL −1 ) over several time frames (0–24 h). The phosphorylation of signaling molecules in the cell lysates was evaluated via Western blotting. p-JNK, p-STAT-1, p-STAT-3 p-Erk 1/2 , and p-P38 are the phosphorylated forms of JNK, STAT-1, STAT-3, Erk 1/2 , and P38, respectively.

Journal: Marine Drugs

Article Title: Multifunctional Cell Regulation Activities of the Mussel Lectin SeviL: Induction of Macrophage Polarization toward the M1 Functional Phenotype

doi: 10.3390/md22060269

Figure Lengend Snippet: Phosphorylation of JAK/STAT and MAPKs by SeviL in RAW264.7 cells. Cells (5 × 10 5 ) were treated with SeviL (10 μg/mL −1 ) over several time frames (0–24 h). The phosphorylation of signaling molecules in the cell lysates was evaluated via Western blotting. p-JNK, p-STAT-1, p-STAT-3 p-Erk 1/2 , and p-P38 are the phosphorylated forms of JNK, STAT-1, STAT-3, Erk 1/2 , and P38, respectively.

Article Snippet: The anti-phosphorylated STAT-1 mAb was obtained from Abcam (Cambridge, UK), whereas the anti-phosphorylated STAT-3 mAb was acquired from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot